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KMID : 0380619810130020101
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Korean Journal of Food Science and Technology
1981 Volume.13 No. 2 p.101 ~ p.106
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Formation of Fungal Protoplast by the Reaction of Cell Wall Lytic Enzymes
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Abstract
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Osmotically sensitive fungal protoplasts were liberated from the mycelium of several kinds f molds by mixed enzyme system of cellulase from Trichoderma veride TO4 and chitinase from the culture filtrate of Streptomyces sp. 115-5. Relatively higher number of protoplast were released from young mycelium of Zygomycetes strains Than Ascomycetes strains by using 10 mM phosphate buffer (pH 6.0) and 0.6 M NaCl as osmotic stabilizer.
Protoplasts were released through ruptures in the wall, initially at the apices, but later also from old party of the hyphae.
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